Using a CCD Camera Imaging System as a Recording Device to Quantify Human DNA by Slot Blot Hybridization

Using a CCD Camera Imaging System as a Recording Device to Quantify Human DNA by Slot Blot Hybridization

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Slot blot hybridization el falcon boss kit of membrane-immobilized, single-stranded human DNA with the higher primate-specific alphoid probe D17Z1 is routinely used in forensic science to estimate the amount of DNA in biological samples.Typically, a chemiluminescent signal captured on film records the hybridization, and the quantity of the signal is related to the amount of immobilized DNA.Digital imaging using a cooled CCD camera offers an alternate non-film-based method for image acquisition with comparable sensitivity of detection, a greater dynamic range, enhanced capability of data interpretation, and often faster results than film.

In addition, the data support the premise 14765-prb-a01 that more accurate and precise human DNA quantification should be obtained by not assuming a linear response of signal to known standards.Instead, quantity should be estimated using a second-order standard curve (R2 = 0.999).

Finally, a CCD camera imaging system offers versatility for image capture of different signal sources and analysis of samples on a variety of support media.